Generator

Part:BBa_K2070013

Designed by: iGEM UT-Tokyo 2016   Group: iGEM16_UT-Tokyo   (2016-10-14)


Constitutive ecf35 generator

This part contains an ECF35 coding region downstream of a medium rbs(BBa_B0032) and strong promoter (BBa_J23101). The ecf35 generated can activate the ecf30 promoter (BBa_K2070000).


Usage and Biology

Measurement of ECF sigma35 and sigma30 promoter activity.Fluorescence/OD600 of a construct containing both an ECF sigma35 generating device BBa_K2070013 and ECF sigma 35 inducible GFP generating device BBa_K2070005 was compared to that containing only either one of the devices. *** for P> 0.001 indicates an extremely significant difference between the induced and uninduced state.



Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


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Categories
Parameters
None